Physiology & Pharmacology
Najmea Farhadi; Davood Mehrabani; Seyed Ebrahim Hosseini; seyede sara hashemi
Volume 28, Issue 5 , November and December 2021, , Pages 814-825
Abstract
Introduction: Cannabis is psychoactive substance that is abused by millions of people the world. Due to the high consumption of this substance among young people of reproductive age, the present study was performed to investigate the effect of cannabis on the growth of endometrial mesenchymal stem cells(msc) ...
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Introduction: Cannabis is psychoactive substance that is abused by millions of people the world. Due to the high consumption of this substance among young people of reproductive age, the present study was performed to investigate the effect of cannabis on the growth of endometrial mesenchymal stem cells(msc) derived from rats.Materials and Methods: In this experimental study, msc were extracted from the endometrium of rats and after culturing and confirming the mesenchymal nature of the cells by flowcytometry and by expressingCD34 and CD90 and not expressingCD105 markers,in the third passage of cell culture, the effects of cannabis in concentration of100and1000ng/ml were calculated on the growth of these cells within1to8days and the results were analyzed using ANOVA and Tuki tests.Results: Cells isolated from the endometrium adhered to the floor of the cell culture flask24hours after transfer. The mesenchymal nature of these cells was confirmed by the expression of CD90, CD105and non-expression of CD34markers.The results ofcell counts also showed the growth of cells treated with cannabis until the third day of treatment similar to the control group. From the fourth day in the treatment group with a dose of100ng/ml cannabis increased significantly top<0.05and in the treatment group with a dose of1000ng/ml. Third, there was significant decrease compared to the control group and from the fourth day, significant increase was observed atp<0.05.Conclusion: The results showed that the cells isolated from the endometrium were of the msc, and that cannabis probably stimulated the growth of these cells through cannabinoid receptors.